Synthetic oligodeoxiribonucleotides

New Variant of Super-Fast Synthesis on Micro-Scale

iplysn@bionet.nsc.ru

Description

Synthetic oligonucleotides are a powerful tool for the solution of many fundamental problems in molecular biology, genetics and gene therapy. They are also the base for working out a number of new high technologies having wide prospects of practical application.

The Institute of Cytology and Genetics, SB RAS, produces synthetic oligodeoxiribonucleotides (ODRN) qualitatively suitable for conducting a wide range of research work.

Fields of Application

At present, synthetic ODRNs have the following fields of application:

  • science - investigations in molecular biology, genetics and medicine;

  • medicine - diagnostics and therapy of multi-factor diseases, neoplasia, viral and bacterial infections;

  • agriculture - earlier diagnostics of infections in agriculturel animals;

  • ecological monitoring - investigating human populations in ecologically unfavourable areas to find out genetic consequences of contamination caused by anthropogenic impact;

  • criminalistics - identification of family relationship and personality with the method of gene dactyloscopy. Despite the fact that synthetic oligonucleotides are quite available, the problem of obtaining them, especially in preparation amounts (1-100g and more), is still topical.

    Technical-Economic Advantages

    More than 500 synthetic ODRNs, each being 10-40 bonds long, were obtained with this method. 
    The quality of the products is level with world standards. 
    There is an economy in hard currency for purchasing oligonucleotides in foreign firms.

    Fields of Application

  • Science,

  • medicine,

  • agriculture,

  • ecology,

  • criminalistics.

    Level and Location of Practical Realisation

    As primers for PCR in the laboratories of the Institute of Cytology and Genetics, SB RAS: 
    1. In molecular-epidemiological investigations for amplification of control region of mitochondrial human DNA and various regions of 6 human genes; 
    2. for analysing the gene structure of protein S100-? in in-bread mice of different lines; 
    3. insertions in transgenic tobacco plants; 
    4. searching for a number of genes in lower vertebra, 
    5. investigating glutenin genes in different cereals; 
    6. for RT-PCR method;

  • As marked probes and competitive DNA in studying glucorticosteroid regulation of tryptophane gene;

  • in studying the interaction of RpII and basal transcription factors during assembly of pre-initiation complex;

  • as probes for hybridisation and RAPD markers when analysing genomic DNA rearrangements in cereals;

  • for DNA amplification from human archeological remains dated 2500.

  • synthesis of primers with GC-suppressors (up to 40 bonds long) for denatured gradient gel electrophoresis;

  • use of primers for allele-specific and nested PCR. ODRNs were also used by:

  • Limnological Institute, SB RAS, Irkutsk,

  • Institute of Therapy, SB RAMS, Novosibirsk,

  • Institute of Clinic Immunology, SB RAMS, Novosibirsk.

    Patent(s)

    Authorship certificate N 1773916, cl. CO7H 21/00.

    Commercial Proposals

    To be determined during negotiations.

    Contacts:

    Institute of Cytology and Genetics, 
    10 Lavrentiev ave., 
    Novosibirsk, 630090, Russia 
    Phone: +7(383) 333-31-18 
    Fax: +7(383) 333-12-78 
    E-mail: kobzev@bionet.nsc.ru