Institute Cytology and Genetics

Laboratory of Morphology and Function of Cell Structures

Head N.B.Rubtsov, Dr.Biol.Sci.

The complete cycle of the sequential steps of the transport of mRNP particles synthesized in the Balbiani ring genes through the nuclear pore in Chironomus observed by transmission (а-е) and scanning (f-t) electron microscopy. Bottom row (1-5) presents a schematic model of mRNP transport.

Ramonsky 77             

mutant nod4           

Ultrastructure of bacteroid containing tissue from the root nodules of pea supernodulating mutant nod4 and variety Ramonsky 77. Scale: 1:10000. (1 - bacteroid, 2 - peribacteroid space, 3 - the cytoplasm of a plant cell).

The main directions of Laboratory research are toward studies on the organization and function of cell structures, as well as toward the development of new methods for their analysis. Modern achievements in molecular cytogenetics, fluorescence and electron microscopy are widely used at the Laboratory. Development of new methods for generating chromosome and region-specific DNA libraries and multi color banding allowed us to perform detailed analysis of chromosome rearrangements in the case of cell malignization and in studies on chromosome evolution in mammals.

Electron microscopic analysis of the structure of the nuclear pore complexes in the nuclear envelope demonstrated the sequence of their assembly _ disassembly during the cell cycle. A model for the structural-functional organization of the nuclear pore in eukaryotes was suggested. A new unique technique for studying the structural and dynamic reorganization of the nuclear pore in yeast was worked out. Change in all the main components of the nuclear pore during the transport of large RNP molecules from the nucleus to the cytoplasm was identified and demonstrated for the first time.

Electron microscopic and morphometric analyses of nitrogen fixing root nodule tissue from pea (Pisum sativum L.) линий varieties Ramonsky 77, Rondo and their supernodulating nod4 and nod3mutants demonstrated the effect of mutations on the formation of the ultrastructure of bacteroid containing nodule tissue.