A hierarchical-block arrangement of Bsp-repeats
The Laboratory is performing combined basic and applied research relevant to
problems of the variability of the genome and gene structure in eukaryotes in
the course of their macro- and microevolution. The major achievements of the
Discovery of DNA repeated sequences (Bsp-repeats) specific to Canidae that
were involved in the genomic reorganization of species of the Canidae family
(Carnivora) during speciation.
Description of the steps of molecular evolution using modern computer
methods for analysis of primary DNA. Comparative analysis of the structure of
the hierarchically block arranged taxon-specific repetitive DNA from the silver
fox, grey fox, domestic dog and raccoon dog to detect 5 types of subrepeats
common to all the species.
All the 5 variants of subrepeats have diverged from a common ancestral DNA
sequence. The establishment of the complex hierarchical structure of repetitive
DNA took 50-60 million years. In contrast, the time of the emergence of the now
living contemporary species, wolf, dog, fox, arctic fox and other canids, dates
back to 5-10 million years. The major elements of repetitive DNA (subrepeats,
monomers) have emerged before speciation. Therefore, the major steps of
molecular evolution of the taxon-specific repeats preceded speciation in
Identification of the possible mechanisms of structure formation and
informational saturation of DNA molecules of regulatory type exemplified by
increasing complexity of both DNA structure and the potential functional loading
in the different variants of the Bsp-repeats.
Obtainment of the first data about the evolutionary features of the gene
pools of mitochondrial DNA in human populations of Siberia by comparative
analysis of DNA samples from the remains of individuals buried 1,000-2,000 B.C.
with those from contemporary inhabitants of the same geographical site.
Analysis of the spread of the allelic variant of the chemokine CCR5
receptor with deletion (delta32) affecting susceptibility to infection in
numerous populations of the Eurasian territories.
Detection of the polymorphic sites in the gene for the macrophage colony
stimulating factor receptor (c-fms) performing important functions in the
regulation of monocyte/macrophage differentiation and proliferation. Genotyping
of the polymorphic site in the 3' flanking region of the c-fms gene occurring in
the Siberian population indicated that the rare variant is very frequent in the
Europeoid (22-26%), Arctic (20-23%) and Central Asian (31-42%) populations. An
association of the c-fms gene with human response to environmental factors,
radiation in particular, was established.