Institute Cytology and Genetics

Laboratory of The Genetics Bases of Neuroendocrine Regulation

Head N.N.Dygalo, Dr.Biol.Sci.

1. Intact and sham operated
2. Orchidectomized (orx)
3. Orx + testosterone treatment

Testosterone affects the expression of the a2A-adrenergic receptors in the brain

Genetic and developmental psychoendocrinology are the main research areas at the Laboratory. The effects of neurotransmitters on the endocrine function of the gonads and adrenals, the action of steroids on adrenergic receptor densities and affinities, mRNA levels in these receptors, determined by RT-PCR, in the brain, as well as hormone-dependent behavior in adult animals are under study. Long-lasting consequences of stress, ionizing radiation, hormones and chemicals affecting brain neurotransmitter systems during the critical periods of early development are also under study.

The obtained results demonstrated that interference into norepinephrine turnover in the brain during the critical period of development can decrease the manifestation of inherited deficiency of the system and norepinephrine-dependent functions.

The expression of the a2A-adrenergic receptor gene is differentially regulated by testosterone in the brain. The hormone increases the expression of the gene in the cortex and decreases it in the brain stem.

Recently, the group started to use a highly selective approach for inhibiting the expression of the adrenoreceptor genes by oligonucleotides complementary to the mRNAs of these genes, antisense-knockdown for investigating the function of these neurogenes in the adult and developing brain.