9. Laboratory of Cytogenetics.
(1) Staff:
- Ekaterina B. Budashkina, Ph.D., Head of the Laboratory
- Antonina I. Shchapova, Dr.Sci.
- Nina P. Kalinina, Ph.D
- Ludmila A. Kravtsova, Ph.D.
- Tatyana A. Potapova
(2) Subjects of Research:
Cytogenetic aspects of genetic material introgression and
stabilization processes in remote hybridization of cereals.
(3) General Results 1993-1995:
Triticum aestivum and Triticum timopheevi hybrid lines were
studied using C-chromosome banding and biochemical markers. Transfer
of T.timopheevi genetic material to T.aestivum involved complete
chromosome, chromosome arms or segments. Frequencies of chromosomes
substitution were the highest for homoeologous group 2 and lowest for
group 4. Introgressive disease-resistant common wheat lines (F10B1,
2n=42) were characterised by chromosome substitution for homoeologous
group 6. It was determined that chromosomes of the 5th homoeologous
group of wheat and rye contain the Edu genes which control the
process of equational division of univalents. Promoter P(Edu) gene
was located on the rye chromosome 5R and the inhibitor I(Edu) gene
was located on the wheat chromosome 5A. A correlation was found
between the frequency of equational division of univalents in
anaphase I and their non-incorporation into newly formed nuclei
in telophase II.
(4) Publications:
- Badaeva E.D., Budashkina E.B., Badaev N.S., Gill B.S. (1993).
Genotype-environment interaction and the process of karyotype
formation: Hybrids between Triticum aestivum x T.timopheevi. In: Proc.
Int. Symp. on Chromosome Engineering in Plants. Columbia, MO,
pp.212-217.
- Shchapova A.I., Potapova T.A., Kravtsova L.A. (1994). The role
of rye genotype in postgamic incompatibility and fertility of
wheat-rye hybrids. Genetika 30:373-376. (Rus.)
- Stepanenko I.L., Budashkina E.B., Kalinina N.P. (1995).
Nitrogen-fixing ability of common wheat introgressive lines with
Triticum timopheevi genetic material. In: Proc. of the 9th EWAC Conf.,
pp.131-133.
- Shchapova A.I., Silkova O.G., Kravtsova L.A. (1995). The role
of chromosoes of the 5th homoeologous group of wheat and rye in the
control of equational division of univalents. Genetika 31:390-395.
(Rus.)
(5) Participation in State and International Programs:
New Trends in Genetics
(6) Subjects of Scientific Collaboration:
Mapping of Triticum timopheevi genetic material introduced into
the common wheat genome.
There is a great collection of introgressive stable common wheat
lines (2n=42) with Triticum timopheevi genetic material. Some of them
were analysed using biochemical markers and differential staining of
chromosomes. A lot of them are disease resistant. Our collection may
be used for isolation of molecular markers (RAPD, PDFR) and mapping of
disease resistance genes. Our preliminary study has shown that some
part of Lr-genes was effectively non-allelic to well known genes of
this type.
We'd like to collaborate with Dr. Beat Keller from Department
of Plant Breeding, Swiss Federal Research Station for Agronomy
(Rochenholzstrasse, 191, ch-8046, Switzerland) and Prof. R.A. Mc
Intosh from Plant Breeding Institute, The University of Sydney,
Australia.