6. Laboratory of Cells Differentiation.
(1) Staff:
- Sergei I. Baiborodin, Ph.D., Executive Head of the Laboratory
- Victoria I. Deribas, Ph.D.
- Vladimir H. Zimmermann, Ph.D.
- Nina V. Baginskaya
- Nina K. Eriskovskaya
- Elena U. Bolobolova
(2) Subject of Research:
- Cell skeleton and differentiation.
- Exogenous DNA-cell interactions.
- Electron microscopy of nucleotide complexes.
- Carcinogenesis and precancer resistance.
(3) General Results 1993-1995:
Using light and electron microscopy and immunocytochemistry for
the study of monoclonal antibodies against glucoso-6-phosphate
dehydrogenase (G6PD) a possibility of G6PD to bind with the elements
of actin cytoskeleton of various types of cells has been shown in
experiment for the first time. This supports a recently developed
conception on the relationship among the enzymes participating in
basal cells metabolism and the elements of cell skeleton. The former
relationship may be treated as a universal cell mechanism providing
compartmentalization of basal processes of biosynthesis in cells.
The relationship between the exogenous DNA (oligonucleotides of
different modifications) and human cells were studied by light and
electron microscopy using biotinstreptovidin-gold labeling. It has
been shown that pT16 oligonucleotide complex passes into the nucleus
and accumulates in it already within 10-15 minutes.
Visualization of oligonucleotides by means of
streptovidinfluorescein labeling has indicated to presumable binding
of this complex at the sites of local single DNA strands. A
distribution pattern of these sites has been obtained.
Study of splicing proteins distribution on mRNA transcripts of
Balbiani ring genes has shown that: a) the proteins which participate
in splicing events bind with RNA at the intron localization sites and
do not arranged all along the transcriptional site; b) splicing
proceeds within 5-6 minutes and can be finished well before or after
transcription is over, dependent on, whether the introns are localized
in the proximal or distal part of the gene.
Active processes of reliable reduction and decrease of
cardiomyocytes number have been recently revealed on the model of
mature rats repeated starving and feeding. Exhaustion of proliferation
potential of population was established at the end of experiment, just
when the mechanisms of cell hypertrophy switch on into the
supplementation of cardiac muscular cells restoration. A new
preparative method of cardiac muscular cell lesions visualization
using luminescent photochemistry has been established and the morphology of
early necrobiosis in cardiomyocytes was studied. A complex of
morphohistochemical methods has been developed, which reliable labels
precancer lesions, not yet affecting the viability, but indicating to
carcinogenic load on animals. This method can be applied in
onco-ecological monitoring of polluted regions.
(4) Publications
- Zakiyan S.M., Nesterova T.B., Tcheryauckene O.V., et al. (1993).
Localization of G6PD in the fibroblasts of field vole and myoblasts of
rat using monoclonal antibodies against G6PD. Cytology 35:81-85.
(Rus.)
- Baiborodin S.I., Baricheva E.M., Bogachyov S.S., et al.
(1993). Betta-heterochromatin of Drosophila: molecular organization
and functioning. Cloning and molecular-biological study of lambda 20
DNA fragment of Drosophila melanogaster betta-heterochromatin.
Genetika 29:403-416. (Rus.)
- Ishigami T., Schmidt-Westhausen A., Philipsen H.P., et al.
(1995). Oral manifestation of alpha-mannosidosis: report of a case
with ultrastructural findings. J. Oral Pathol. Med. 24:85-88.
- Yakubov L.A., Nechaeva M.V., Andreeva A., et al. (1995).
Nucleic-acid binding receptors and oligonucleotides traffican in cells
and organism. In: France-Russian Symp. "Regulation of Gene
expression". Novosibirsk, July 1-5, 1995, p.42.
- Nepomnyashchikh L.M., Semyonov D.E., Tsimmerman V.G. (1994).
Influence of repeated starving on numerical traits of cardiomyocytes
population in the white rat. Bull. Experimental Biology and Medicine
N6:658-660. (Rus.)
- Tsimmerman V.G. (1994). Morphological study of early
necrobiosis in cardiac muscular cells using fluorochrome
photochemistry. In: Abstr. of cand. dissert. in biology. Novosibirsk.
16 p.
- Karakin E.I. Ronichevskaya G.M., Deribas V.I., et al. (1995).
Genetical monitoring of natural populations of rodentia in regions
differing in levels of anthropogenic factors. In: The II-nd
Vereshchagin Baikal Conf. Oct. 5-10, 1995. Irkutsk, p.77.
- Deribas V.I., Galieva E.R. (1995). Carcinogenic monitoring of
environment assessed by precarcinogenic lesions in the organs of the
wild rodentia populations. In: Theses of the IInd Vereshchagin Baikal
Conf., Oct. 5-10, Irkutsk.
- Kaledin V.I., Deribas V.I., Morozkova T.S. Frequency
variability of liver tumor in the reciprocal hybrids of mice. Reports
of the Acad. Sci. (in press).
- Deribas V.I. (1995). Cases of normalization of menstrual cycle
by means of natural medicaments. In: XV Eur. Congr. of Pathol.
Copenhagen, Denmark, Sept. 3-8, presentation 24.
- Eriskovskaya N.K., Kerkis A.Yu., Solovyova N.A., Salganik R.I.
(1994). Hereditary hyperproduction of free radicals. Induction of
carcinogenesis. Rep. of the Acad. Sci. 338:255-258.
(6) Subjects of Scientific Collaboration:
- Study of tissue resistance to carcinogenesis.
Collaboration is possible in the field of neoplasm morphology and
distribution in the organs of wild rodentia populations and under
conditions of induced carcinogenesis. This work will significantly
improve the efficiency of assessment of the carcinogenic load on
environment.
- Study on exogenous nucleic acids entrance into mammalian cells.
Collaboration is possible in working out efficient methods of
polyand oligonucleotides introduction into cell culture and into cells
of the living organisms, which is necessary for working out new
research vectors in molecular biology and therapy (gene therapy,
establishment of biological functions of definite genes etc.).